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texasred  (Wuhan Fine Biotech)

 
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    Wuhan Fine Biotech texasred
    Texasred, supplied by Wuhan Fine Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/texasred/labeled+texasred/pmc12524445-173-5-10
    Average 86 stars, based on 1 article reviews
    texasred - by Bioz Stars, 2026-10
    86/100 stars

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    other:

    Article Title: Stefin A Regulation of Cathepsin B Expression and Localization in Cancerous and Non-Cancerous Cells
    Article Snippet: CTSB was detected using a TexasRed-labeled secondary antibody (FineTest Corp. Wuhan Fine Biotech Co., Ltd., Wuhan, China).



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    (a) Western blot analysis and quantification of TfR protein expression in hG2019S-transgenic (hG2019S tg), Lrrk2-deficient (Lrrk2 k.o.) and wild-type fibroblasts (n = 8 independent experiments). (b) <t>Transferrin</t> recycling assay in rat fibroblasts: Phalloidin (green), DAPI (data not shown) and TexasRed transferrin (white) staining were used for automated quantification of generated data (segmentation and ROI count). Representative images for wild-type fibroblasts, time points 0 und 70 min (top). Graphical representation of transferrin uptake at different time points (= incubation time) in hG2019S tg (left, n = 11 independent experiments) and Lrrk2 k.o. (right, n = 9 independent experiments) fibroblasts (button). (c) TfR protein degradation at different time points after cycloheximide addition (n = 8 independent experiments). (d) Western blot analysis/ quantification of Rab12 expression in hG2019S tg, Lrrk2 k.o. and wild-type fibroblasts.
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    (a) Western blot analysis and quantification of TfR protein expression in hG2019S-transgenic (hG2019S tg), Lrrk2-deficient (Lrrk2 k.o.) and wild-type fibroblasts (n = 8 independent experiments). (b) Transferrin recycling assay in rat fibroblasts: Phalloidin (green), DAPI (data not shown) and TexasRed transferrin (white) staining were used for automated quantification of generated data (segmentation and ROI count). Representative images for wild-type fibroblasts, time points 0 und 70 min (top). Graphical representation of transferrin uptake at different time points (= incubation time) in hG2019S tg (left, n = 11 independent experiments) and Lrrk2 k.o. (right, n = 9 independent experiments) fibroblasts (button). (c) TfR protein degradation at different time points after cycloheximide addition (n = 8 independent experiments). (d) Western blot analysis/ quantification of Rab12 expression in hG2019S tg, Lrrk2 k.o. and wild-type fibroblasts.

    Journal: bioRxiv

    Article Title: The Parkinson’s disease associated Leucine-rich repeat kinase 2 affects expression of Transferrin receptor 1 and phosphorylation of key signaling proteins in human iPSC-derived dopaminergic neurons

    doi: 10.1101/2025.05.06.652393

    Figure Lengend Snippet: (a) Western blot analysis and quantification of TfR protein expression in hG2019S-transgenic (hG2019S tg), Lrrk2-deficient (Lrrk2 k.o.) and wild-type fibroblasts (n = 8 independent experiments). (b) Transferrin recycling assay in rat fibroblasts: Phalloidin (green), DAPI (data not shown) and TexasRed transferrin (white) staining were used for automated quantification of generated data (segmentation and ROI count). Representative images for wild-type fibroblasts, time points 0 und 70 min (top). Graphical representation of transferrin uptake at different time points (= incubation time) in hG2019S tg (left, n = 11 independent experiments) and Lrrk2 k.o. (right, n = 9 independent experiments) fibroblasts (button). (c) TfR protein degradation at different time points after cycloheximide addition (n = 8 independent experiments). (d) Western blot analysis/ quantification of Rab12 expression in hG2019S tg, Lrrk2 k.o. and wild-type fibroblasts.

    Article Snippet: To investigate the impact of TfR downregulation on its internalization, we observed the TfR translocation by Transferrin Recycling Assay using the TexasRed-labeled transferrin and BD Pathway 855 imaging system in the same rat cell lines.

    Techniques: Western Blot, Expressing, Transgenic Assay, Staining, Generated, Incubation